English translation for "host strain"

寄助株

Related Translations:

host material:  基质材料，晶核材料晶核材料

event host:  司仪

host compound:  主体化合物

remote host:  远程主机远程助

host resident:  主机常驻驻在主机系统

intermediate host:  【生物学】中间宿主。

host forum:  社区-主机论坛

host area:  移民安置区

host id:  网络中某台机器的因特网地址也称为主机标识主机标识

host range:  寄侄围宿主范围

Example Sentences:

	1.	Two main parts were included in the present thesis . part i . cloning and expression cryigenes of bacillus thuringiensis in different host strains 一、苏云金芽胞杆菌杀虫晶体蛋白基因在不同受体菌中的表达1
	2.	It was then cloned to the secreted vector - ppic9k and recombined successfully into the chromosome of pichia pastoris host strain - gsl 15 by electroporation 通过电转化作用该基因片段被成功地整合至酵母cs115的染色体上，经过甲醇诱导之后，该基因得到了分泌表达。
	3.	Transformation was done by electroporation . human fl extracellular domain cdna transformed to yeast host strain km71 , then his + muts phenotype transformant was screened out and cultured in flasks , and rhfl was expressed under the induction of 0 . 5 % methanol 我们提取了km71ppic9k - fl转化菌株的基因组dna进行southern实验，检测目的基因的整和插入；提取总rna ，进行了northern实验，检测fl基因在转化菌株中的表达。
	4.	A gene library of psedomonas fluorescens g2 was constructed in the cosmid vector pla2917 using e . coli jm109 as the host strain . two recombinants , pgr3 and pgr7 , which can confer glyphosate resistance ofe . coli jm109 were identified from the selective medium containing 10mm glyphosate 以粘粒pla2917为载体、大肠杆菌jm109为受体菌构建荧光假单胞菌g2的基因组文库，在含有10mm草甘膦的固体选择培养基上筛选出两个耐受克隆pgr3和pgr7 ，插入片段分别为7kb和11kb 。
	5.	The recombination vectors were transformed into host strain of bl21 and induced with iptg . all the recombinant protein was expressed into inclusion body . the recombinant protein was identified with sds - page and westen - blot and purified through elution method . the muticlone antibody was got by immuning the rabbit with the purified protein 把重组质粒转化入表达受体菌bl21 ，经iptg诱导后都获得了表达，且都以包涵体的表达形式存在，用sds - page和western - blot的方法对重组蛋白进行了鉴定。
	6.	The hwtx - i gene was chemically synthesized according to its known cdna sequence , the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor , the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis , then it was transformed into host strain gs115 , a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations , the multicopy transformant was named gh1 . gh1 was cultivated in flasks . after 6 days of induction by 0 . 5 % methanol , the supernatant was checked by 16 . 5 % tricine - sds page , which showed there was a band in the position of 3 . 5 - 6 . 1kd , then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column , after reverse phase hplc of ci8 and vacuum drying , the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page , maldi - tof mass spectrometry , amino acid composition analysis , the n - terminal amino acid sequence and its biological activity , the final field of the purified rhwtx - i was about 80mg / l , accounting for 23 . 6 % of it total secretory proteins 将带有hwtx -基因的ppic9k经blgii线性化后，转化酵母宿主菌gs115原生质体后经筛选阳性克隆并经表型鉴定为his ~ + mut ~ s酵母菌，进一步用遗传毒素g418筛选多拷贝的转化菌株，命名为gh1 ；将gh1甲醇酵母菌用0 . 5的甲醇诱导表达，发酵上清经90饱和度的( nh _ 4 ) _ 2so _ 4沉淀， yw - 3 ( mwc03000 )的超滤膜超滤，再经cm阳离子交换， c _ ( 18 )反相hplc纯化得到分子量为4kd左右的组分，其中4289 . 05的组分经质谱鉴定，氨基酸组成分析和序列测定为正确的表达产物，生物学活性表明其活性为天然毒素活性70 % ，表达量为80mg / l 。
	7.	The fragments were ligated directly to the pichia pastrois expression vector ppic9 to got ppic9 - e3and ppic9 - e8 . vectors were amplificated in the e . coli dh5 a and were linearized with bgl ii . the linearized vctors were transformed into host strain gs115 . the recombinated strain was selected though phynotype and pcrthe positive strain was induced with methyl alcohol and was selected by dot - elisa . the recombinated protein was detected with sds - page and western - blot as before 重组菌用甲醇诱导表达，用dot - elisa的方法筛选到表达量较高的菌株。将筛选出的菌株大量的诱导表达，对表达上清处理后，用sds - page和western - blot进行鉴定。同时，用hiprep16 10heparinff肝素亲和柱对表达蛋白进行了初步的纯化。

Similar Words：

"host software version" English translation, "host species" English translation, "host specific" English translation, "host specificity" English translation, "host spectrum" English translation, "host structure" English translation, "host subarea" English translation, "host subscriber" English translation, "host suitability" English translation, "host support" English translation